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The pellets were resuspended in nuclear lysis buffer (50 mM Tris HCl pH 8.1, 10 mM EDTA, 1% w/v SDS)

170 Misfolded proteins targeted by RNF5 include mutant CFTR, 119 solute carrier family 1 member 5 (SLC1A5), and SLC38A2

In addition, NAC helps to support healthy brain and neuronal tissues

Maintaining adequate levels of glutathione can support overall health and improve the body's detoxification ability

These metabolites were grouped into lipid-metabolism molecules such as cholestenone, cholesterol, 3 methyladipate pimelate and X2 hydroxymyristic acid, active metabolites and neurotransmitters such as valeric/isovaleric acid, azelate, azelaic acid, C20:4 carnitine, undecanedioic acid, vitamin related metabolites such as the pyridoxamine and nucleotide/nucleoside metabolites such as the deoxyinosine